One-of-a-kind study reveals germination of spore-forming probiotic in gastrointestinal tract


Of course, to be effective, probiotics must reach their target location alive. BacillusIs a sporulating bacteria and confers many advantages over probiotic strains of lactic acid bacteria. In their spore form, they are able to survive the harsh gastric environment and reach the small intestine alive.

Spores B. subtilisMay themselves modulate the host’s immune response (Huang et al., 2008); however, the full potential of spore-based probiotics can only be achieved if they also germinate and become active vegetative cells in the small intestinal tract.

Confirm the presence of BacillusSpores and vegetative cells in the small intestinal tract are difficult. An unexplored approach is to analyze the ileal effluent (the part of the small intestine that absorbs nutrients) from healthy participants who have had an ileostomy. An ileostomy is a surgical procedure in which the end of the ileum is passed through an opening in the abdomen known as an ileal stoma. An ostomy bag is connected to the stoma where all of the intestinal contents are collected.

The present study aimed to study the survival capacity and germination of B. subtilisProbiotic strain DE111 (supplied by Deerland) in the small intestine by ileostomy.

The randomized, crossover, double-blind, placebo-controlled study involved 11 individuals (aged 24 to 75 years) with stable ileostomies.

With access to the contents of ileal pouches, researchers were able for the first time to directly examine spore germination under in vivo conditions in real time in the human small intestine.

In the study, participants with ileal pouches consumed 5 billion CFU of DE111 or a placebo with a standardized meal; each participant took a placebo first, went through a week-long washout, and then took DE111.

The contents of their ileal pouches were collected hourly after consumption for eight hours, and the DE111 spore and vegetative cell counts were studied. After consuming DE111, all participants showed both DE111 spores and DE111 vegetative cells present in their ileostomy bags. This was not the case after taking the placebo.

During the 8 hour study period – the average time for food to pass completely from the mouth to the small intestine – the combined number of DE111 spores and DE111 vegetative cells emanating from the ileum was equal to or greater the number of spores consumed at the start of the study. Researchers say this indicates excellent survivability of DE111, as well as the strain’s growth and reproduction in the digestive tract.

“The germination of spore-forming probiotics in the small intestine is of particular importance since a significant part of the immune system is located in this part of the intestine and the majority of digestion and absorption of nutrients occurs there. ” explained John Deaton, vice president of science and technology for Deerland Probiotics & Enzymes. “Prior to this new study, others attempted to determine spore germination in the small intestine using simulated laboratory models or animal studies, but none investigated actual spore germination in vivo. in the human small intestine. “

Deaton added, “This study provides clear evidence that DE111 spores germinate in the human small intestine. This then makes it possible to show that the consumption of B. subtilis DE111 effectively promotes and supports immune and digestive health.

Source: Frontiers in microbiology

Colom J, Freitas D, Simon A, Brodkorb A, Buckley M, Deaton J and Winger AM

Presence and germination of the probiotic Bacillus subtilis DE111 in the human small intestine “

doi: 10.3389 / fmicb.2021.715863


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